Spheroids by Seeding Stem Cells in Bins to Allow Aggregation to Occur Before Harvesting

This video rendering depicts the way I make stem cell spheroids. We add stem cells (purple) in suspension into a well containing inverted pyramidal bins (microwells) that physically separate the cells. Over time, the cells within each microwell will interact with each other and condense (compact) into a dense, cellular spheroid. In this schematic, I show (9) spheroids forming at once but in reality we form hundreds of thousands of spheroids at once to meet the demand of performing experiments. This process takes about 2 days but is highly dependent on the cell type, source and cell density initially added into the bins. Spheroid size can be controlled through cell density as well.